b. Peptones as nitrogen source
c. Carbohydrates as carbon source
d. Minerals (Metals and inorganic salts)\
e. Selective agents
g. Dyes and pH indicators
h. Solidifying agent
2. Carbohydrates used in culture media are tested to ensure their identity and purity from adulterants. Growth supportive properties of carbohydrates are tested by preparing liquid and solid culture media along with known standard carbohydrates. On solid media, colony characteristics on reference and test carbohydrate media are compared after sufficient incubation period for size, colour, shape and enzyme reaction with indicators.
At HiMedia, we have made ‘Quality’ possible by maintaining consistent Quality systems in the Design as well as Production phase. Further to enhance production facilities since last 3 years we have introduced WHO GMP at every stage to ensure “Quality” of products manufactured. Our motto “in the service of mankind” has remained the same since our establishment in 1976.
Therefore, we analyze the dyes used in our culture media as specified by H.J. Conn’s. Besides ascertaining chemical purity of dyes, microbiological suitability is carried out by adding dyes in Culture Media. These are then tested with various test organisms and compared with standard known dyes.
Various chemical impurities are analyzed while testing agar for bacteriological purpose. Besides above tests, toxicity test is carried out to ascertain the presence of toxic and inhibitory substances. This is done by growing fastidious organisms on culture media where agar is incorporated and the growth, colony size, shape, pigments, etc. are compared with known standard agar.